In Vitro Effects of Ethanol and Aspirin Combined Administration on Erythrocyte Membrane Properties Eri̇trosi̇t Membraninda Etanol Ve Aspi̇ri̇ni̇n Bi̇rli̇kte Veri̇lmesi̇ni̇n İn Vi̇tro Etki̇leri̇

This report describes in vitro effect of ethanol and combined administration of ethanol with aspirin on erythrocyte membrane properties. Furthermore, we evaluated the possible protective role of sAdenosylmethionine (SAMe) and for this aim, we determined plasma malondialdehyde (MDA), sialic acid (SA) levels and the activity of membrane-bound Na-KATPase. Concentration doses were adjusted as control (0% ethanol), ethanol (0,5 %), aspirin (4 mg/ml) and SAMe (2.5 nM). Blood samples were divided into five groups as control, ethanol, [ethanol+aspirin], [ethanol+SAMe] and [ethanol+SAMe] group and incubated on 25°C for 16 h. After this, we measured Na-K ATPaz, MDA, Sialik Asit levels. Na-KATPase activities were decreased in the ethanol and 0,5% [ethanol+SAMe] groups as compared to the control group (p<0.05). Plasma MDA levels in the [ethanol+aspirin] and [ethanol+aspirin+SAMe] groups were higher than in the control group (p<0.001), also the [ethanol+aspirin] group was higher than in the ethanol group (p<0.001). SA levels in the [ethanol+SAMe] groups were increased significantly as compared to the control group (p<0.01). We assume that aspirin protected ethanol-induced inactivation of Na-K ATPase activities and also decreased sialic acid levels. But, combined administration of aspirin with ethanol increased peroxidative injury of erythrocyte membranes. We think that protective role of SAMe may be dosedependent. (Pam Med J 2009;2(1):1-7).